TILAPIA FISH (Kerala University of Fisheries and Ocean Studies (KUFOS))

The Kerala University of Fisheries and Ocean Studies (KUFOS) conducted a comprehensive study to evaluate the impact of Nanobubble Oxygen (NB-O2) Technology on fish welfare, water quality, and overall growth performance in Nile Tilapia (Oreochromis niloticus).

Objectives

To evaluate the impact of NICO’s Nanobubble Oxygen (NB-O2) Technology on the welfare, growth, and water quality in Nile Tilapia over a 3-month period.

Key Findings & Benefits Of Nico Nanobubble Technology In Aquaculture

  • Improved Dissolved Oxygen (DO) Stability
  • Enhanced Growth Performance & Feed Efficiency
  • Reduced Stress & Improved Fish Welfare
  • Better Water Quality & Waste Management

Methodology

Study Groups:
  • Two groups: Control (air treatment) and NB-O2 treatment.
  • Each group had 3 replicates, with 30 fish per tank.
  • NB-O2 was applied for 10 minutes, twice daily, for 3 months.
Fish Details:
  • Nile Tilapia fingerlings (avg. 6.98 ± 0.04 g) sourced from RGCA hatchery, Kochi.
  • Fish were acclimated for 2 weeks.
  • Fed 28% protein diet, twice daily at 2% of body weight.
  • Health screening was done before the experiment to exclude infected fish.
Data Collection:
  • Growth (weight & length) was measured weekly on 10 randomly selected fish per tank.
  • One fish from each group was sacrificed monthly for blood sampling.
  • Remaining fish returned to tanks after handling.
Lab Analysis:
  • Haematology via BC-30 Vet (Mindray).
  • Serum metabolites & enzymes via Dri-Chem Nx500V.
  • Oxidative stress markers tested with Origin kits.
  • Cortisol measured using ELISA (Cusabio, USA).

Results

NB-O2 Group Control Group
Initial BW 6.98±0.04 6.98±0.04
Final BW 12.75±0.65* 8.74±0.41
% weight gain 82.72% (5.77g) 25.22% (1.76g)
Growth rate 0.064g/day 0.0196g/day
Specific Growth rate 0.67 %/day 0.25%/day
Cortisol Level 10.20±1.31mg/mL 19.26±1.17mg/mL

Conclusion

NICO's NB-O2 tech significantly enhances aquaculture productivity, fish health, and sustainability. It represents a transformative, scalable solution for modern fish farming.

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